DNA array analysis of altered gene expression in human leukocytes stimulated with soluble and particulate forms of
Ken-ichi Ishibashi1, Noriko N Miura, Yoshiyuki Adachi
1Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
Abstract:
We previously reported that 1,3-beta-d-glucan derived from Candida albicans, a pathogenic fungus was obtained by oxidation of the cell wall with sodium hypochlorite (NaClO). It could be solubilized by treatment with dimethylsulfoxide (DMSO). We found that OX-CA and CSBG showed significantly different levels of activity toward leukocytes. Here, we have used cDNA microarrays to analyze the mRNA expression of 1176 genes in PBMCs stimulated with Candida cell wall glucan and considered the difference in the activation mechanism of OX-CA and CSBG. Total mRNA showed a significant change for 147 out of 1176 arrayed genes on stimulation with OX-CA and CSBG for 4 h. Among those genes, 62 were common, 26 were OX-CA-specific and 59 were CSBG-specific. Many of these up-regulated genes encode effectors with well-characterized proinflammatory properties. The expression of genes related with signal transduction differed in the particulate and soluble glucans derived from C. albicans having exactly the same primary structure. This fact suggested that each glucan induced specific biological activity through a different activation mechanism. This study using cDNA microarrays to analyze a broad spectrum of mRNA expression provides information on the biological activity of Candida cell wall glucan as a potential pathogenic factor.
