Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Coupling morphogenesis to mitotic entry.

Krisada Sakchaisri1, Satoshi Asano, Li-Rong Yu

  • 1Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Building 37, Bethesda, MD 20892, USA.

Proceedings of the National Academy of Sciences of the United States of America
|March 24, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lorlatinib protects dopaminergic neurons by inhibiting ALK-mediated neuroinflammation in a mouse model of Parkinson's disease.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

Plantago lanceolata extract inhibits porcine epidemic diarrhea virus and emerging enveloped viruses by interfering with virus internalization in vitro.

Journal of ethnopharmacology·2026
Same author

Reclassification and Recombination Analysis of Porcine Epidemic Diarrhea Virus Strains in South Korea Based on Spike Gene Analysis.

Veterinary sciences·2026
Same author

Corrigendum to "Activation of α7nAch receptors ameliorates α-synuclein pathology in the brain and gut of a subacute MPTP mouse model of Parkinson's disease" [Biomed. Pharmacother. 184 (2025) 117871].

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

Association between number of teeth and oxidative balance score in Korean adults: a population-based study.

Korean journal of community nutrition·2026
Same author

Mutant KRAS in brain endothelial cells promotes vascular inflammation and impairs vascular integrity in brain arteriovenous malformation.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism·2026

Budding yeast Swe1 (Wee1 ortholog) stabilizes at the bud neck, delaying mitosis. Cla4/PAK and Cdc5/Polo kinases sequentially phosphorylate and degrade Swe1, linking cell structure to mitotic entry.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Cyclin-dependent protein kinase 1 (CDK1) entry into mitosis is regulated by Wee1 protein kinase.
  • In budding yeast, timely mitotic entry depends on Swe1 (Wee1 ortholog) inactivation.
  • Septin collar perturbations stabilize Swe1, delaying the G2/M cell cycle transition.

Purpose of the Study:

  • To investigate the kinases responsible for Swe1 phosphorylation and degradation at the bud neck.
  • To elucidate the mechanism linking cellular structure assembly to mitotic progression.

Main Methods:

  • Yeast genetics and cell biology techniques.
  • Analysis of protein localization and phosphorylation.
  • Ubiquitin-mediated protein degradation assays.

Related Experiment Videos

Main Results:

  • Swe1 is recruited to the bud neck and hyperphosphorylated, leading to its degradation.
  • Hsl1 kinase is required for Swe1 neck localization but not its phosphorylation.
  • Cla4/PAK and Cdc5/Polo kinases sequentially phosphorylate Swe1 at the bud neck, promoting its down-regulation.

Conclusions:

  • Cla4/PAK and Cdc5/Polo kinases orchestrate stepwise Swe1 phosphorylation and degradation.
  • This process links septin ring assembly to the G2/M transition, ensuring proper cell division.