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Demonstrating internal ribosome entry sites in eukaryotic mRNAs using stringent RNA test procedures.

Marc E Van Eden1, Marshall P Byrd, Kyle W Sherrill

  • 1Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas 77030-3498, USA.

RNA (New York, N.Y.)
|March 24, 2004
PubMed
Summary

Aberrant splicing in dicistronic DNA assays can inflate internal ribosome entry site (IRES) activity measurements. Combining RNAi and RT-PCR effectively detects these aberrant RNAs, improving IRES analysis accuracy.

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