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Published on: February 10, 2020
Determination of morphine by capillary zone electrophoresis immunoassay combined with laser-induced fluorescence
Jian-Qiu Mi1, Xin-Xiang Zhang, Wen-Bao Chang
1The Key Lab of Bioorganic Chemistry and Molecular Engineering, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing, PR China.
Abstract:
A competitive immunoassay for detecting morphine in bio-samples was established by capillary zone electrophoresis combined with laser-induced fluorescence detection (CZE-LIF). The antigen of morphine was labeled with isothiocyano-fluorescein (FITC) and then incubated with morphine monoclonal antibody and samples. The linear range was 50-1000 ng/mL, which was suitable for clinical and forensic applications. The detection limit can reach 40 ng/mL, based on S/N = 2. The recoveries of morphine from serum were satisfactory.
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