Related Experiment Videos
In vitro analysis of histone acetyltransferase activity
Laura J Benson1, Anthony T Annunziato
1Biology Department, Boston College, 140 Commonwealth Ave., Chestnut Hill, MA 02467, USA.
Methods (San Diego, Calif.)
|March 25, 2004
Abstract:
Interest in histone acetylation has risen dramatically in recent years. In the following report, we present methods for the analysis of histone acetyltransferase activity in vitro. Protocols are described for radiolabeling histone proteins and N-terminal "tail" peptides, and for the analysis of acetylation by immunoblotting. Techniques for acetylating immobilized histone peptides are also described.