Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Biosynthesis of Lipids01:29

Biosynthesis of Lipids

702
Microbial membranes exhibit remarkable diversity in lipid composition, reflecting evolutionary adaptations to various environmental conditions. The three domains of life—Bacteria, Archaea, and Eukarya—synthesize membrane lipids through distinct biosynthetic pathways, leading to fundamental structural differences that impact membrane stability, function, and adaptability.Fatty Acid-Based Lipids in Bacteria and EukaryaBacteria and eukaryotes share a common fatty acid biosynthesis...
702
Biosynthesis of Nucleic Acids01:28

Biosynthesis of Nucleic Acids

1.2K
Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
1.2K
Amino Acid Biosynthetic Pathways01:29

Amino Acid Biosynthetic Pathways

1.4K
Amino acid biosynthesis is essential for cell growth, protein synthesis, and metabolic regulation. Cells generate essential and non-essential amino acids from metabolic intermediates to sustain vital biological functions. These intermediates originate from key metabolic pathways: glycolysis, the tricarboxylic acid (TCA) cycle, and the pentose phosphate pathway. Important precursors include α-ketoglutarate, pyruvate, oxaloacetate, phosphoenolpyruvate, and erythrose-4-phosphate, which...
1.4K
Biosynthesis of Polysaccharides01:26

Biosynthesis of Polysaccharides

730
Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...
730
Biosynthesis in Bacteria01:24

Biosynthesis in Bacteria

773
Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
773
Synthetic Biology02:55

Synthetic Biology

5.6K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
5.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DVD-IgG1 antibody-drug conjugates: Expanding the landscape of targeted cancer therapy.

Current opinion in chemical biology·2026
Same author

Discovery of 5‑Chlorotryptophan-Containing Antibiotics through Metabologenomics-Assisted High-Throughput Screening.

JACS Au·2025
Same author

Chemical Tagging of <i>N</i>-Alkylamine-Containing Natural Products and Pharmaceuticals through C(<i>sp</i><sup>3</sup>)-H Functionalization.

Journal of the American Chemical Society·2025
Same author

Logical Exploration of Cinnamoyl-Containing Nonribosomal Peptides via Metabologenomic Targeting and Regulator Overexpression.

Journal of the American Chemical Society·2025
Same author

Second Generation Tiancimycin-Based Antibody-Drug Conjugates Enabled by Highly Efficient Semi-synthetic Approach Specifically Targeting B-Cell Malignancies.

JACS Au·2025
Same author

Coenzyme A Tethering Mediates Dehydratase Substrate Promiscuity and Reaction Specificity in Platensimycin, Platencin, and Platensilin Biosynthesis.

Biochemistry·2025

Related Experiment Video

Updated: Feb 17, 2026

Mass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products
11:13

Mass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products

Published on: March 12, 2020

11.6K

Accessing natural products by combinatorial biosynthesis.

Ben Shen1

  • 1Division of Pharmaceutical Sciences and Department of Chemistry, University of Wisconsin-Madison, 777 Highland Avenue, Madison, WI 53705, USA. bshen@pharmacy.wisc.edu

Science'S STKE : Signal Transduction Knowledge Environment
|March 25, 2004
PubMed
Summary
This summary is machine-generated.

Combinatorial biosynthesis enhances production of phoslactomycin B, a protein phosphatase IIa inhibitor. This engineered pathway offers a promising method for creating complex natural products and their analogs.

More Related Videos

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
07:59

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

10.4K
From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes T&#252;6028
09:08

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

Published on: January 13, 2017

17.8K

Related Experiment Videos

Last Updated: Feb 17, 2026

Mass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products
11:13

Mass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products

Published on: March 12, 2020

11.6K
A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
07:59

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

10.4K
From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes T&#252;6028
09:08

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

Published on: January 13, 2017

17.8K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Natural Product Synthesis

Background:

  • Phoslactomycin (PLM) B is a bioactive natural product with protein phosphatase IIa inhibitory properties.
  • The PLM biosynthetic pathway presents a target for metabolic engineering to improve production.
  • Complex natural products and their analogs are valuable in drug discovery.

Purpose of the Study:

  • To enhance the production of phoslactomycin B.
  • To selectively produce phoslactomycin B using a rational engineering approach.
  • To demonstrate the utility of combinatorial biosynthesis for natural product analog generation.

Main Methods:

  • Rational engineering of the phoslactomycin biosynthetic pathway.
  • Application of combinatorial biosynthesis techniques.
  • Fermentation and purification of phoslactomycin B.

Main Results:

  • Successful enhancement of phoslactomycin B production.
  • Selective generation of phoslactomycin B was achieved.
  • Demonstrated effectiveness of the engineered pathway.

Conclusions:

  • Rational engineering of the PLM biosynthetic pathway is effective for enhancing and selectively producing PLM B.
  • Combinatorial biosynthesis is a powerful strategy for the preparation of complex natural products and their analogs.
  • This approach holds promise for the development of novel therapeutic agents.