Related Experiment Video
Updated: Aug 25, 2026
Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
Published on: January 7, 2019
Assembly of Marek's disease virus (MDV) capsids using recombinant baculoviruses expressing MDV capsid proteins
1Laboratoire de Virologie et Barrière d'Espèces, UR086, INRA, Centre de Recherche de Tours, 37380 Nouzilly, France.
Abstract:
The genes UL18, UL19, UL26, UL26.5, UL35 and UL38 of Marek's disease virus 1 (MDV-1) strain RB1B, encoding the homologues of herpes simplex virus type 1 (HSV-1) capsid proteins VP23, VP5, VP21-VP24, preVP22a, VP26 and VP19C, were identified and sequenced. Recombinant baculoviruses were used to express the six capsid genes in insect cells. Coexpression of the six genes or of UL18, UL19, UL26.5 and UL38 in insect cells resulted in the formation of capsids with a large core. In addition, electron microscopy of thin sections clearly revealed the presence of large numbers of small spherical particles. Experimental coinfection demonstrated that these small particles were associated with production of the preVP22a protein.
Insights
Marek
Area of Science:
- Virology
- Molecular Biology
- Genetics
Background:
- Marek's disease virus 1 (MDV-1) is an oncogenic avian herpesvirus.
- Understanding MDV-1 capsid protein assembly is crucial for viral replication and pathogenesis.
Purpose of the Study:
- To identify and sequence key MDV-1 genes encoding capsid proteins.
- To investigate the assembly of MDV-1 capsids using recombinant baculoviruses and insect cells.
Main Methods:
- Sequencing of MDV-1 genes (UL18, UL19, UL26, UL26.5, UL35, UL38).
- Expression of these genes in insect cells using recombinant baculoviruses.
- Electron microscopy to analyze capsid formation and particle structure.
Main Results:
- Six MDV-1 capsid genes were identified and sequenced.
- Coexpression of these genes led to the formation of capsids with large cores.
- Small spherical particles associated with preVP22a protein production were observed.
Conclusions:
- The identified MDV-1 genes encode functional capsid protein homologues.
- Coexpression facilitates the assembly of MDV-1 capsids.
- PreVP22a protein is linked to the formation of specific viral particles.
