Determination of abamectin and doramectin in sheep faeces using HPLC with fluorescence detection
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Lucija Kolar1, Jernej Kuzner, Nevenka Kozuh Erzen
1University of Ljubljana, Veterinary Faculty, Institute of Physiology, Pharmacology and Toxicology, Gerbiceva 60, 1000 Ljubljana, Slovenia. lucija.kolar@vf.uni-lj.si
Abstract:
An analytical procedure for the determination of abamectin and/or doramectin in sheep faeces has been developed. Avermectins were extracted from sheep faeces with acetonitrile, clean-up using solid phase extraction (SPE) and analysed by high performance liquid chromatography (HPLC) with fl uorescence detection after derivatization with N-methylimidazole.The method has a low detection limit (1.0 ng/g of moist sheep faeces), low quanti fi cation limit (2.5 ng/g of moist sheep faeces), good recovery in the range 66.4-80.8% for abamectin and 67.7-85.5% for doramectin as well as good repeatability (>85%). The method is applicable to the study of the time pro fi le of excretion in sheep faeces and also for ecotoxicological studies of both avermectins.
