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Determination of abamectin and doramectin in sheep faeces using HPLC with fluorescence detection
Lucija Kolar1, Jernej Kuzner, Nevenka Kozuh Erzen
1University of Ljubljana, Veterinary Faculty, Institute of Physiology, Pharmacology and Toxicology, Gerbiceva 60, 1000 Ljubljana, Slovenia. lucija.kolar@vf.uni-lj.si
Abstract:
An analytical procedure for the determination of abamectin and/or doramectin in sheep faeces has been developed. Avermectins were extracted from sheep faeces with acetonitrile, clean-up using solid phase extraction (SPE) and analysed by high performance liquid chromatography (HPLC) with fl uorescence detection after derivatization with N-methylimidazole.The method has a low detection limit (1.0 ng/g of moist sheep faeces), low quanti fi cation limit (2.5 ng/g of moist sheep faeces), good recovery in the range 66.4-80.8% for abamectin and 67.7-85.5% for doramectin as well as good repeatability (>85%). The method is applicable to the study of the time pro fi le of excretion in sheep faeces and also for ecotoxicological studies of both avermectins.
