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[Stereoselectivity in absorption of trans tramadol in rat intestine]
Hui-chen Liu1, Bao-xin Li, Bin Dun
1Department of Clinical Pharmacology, Bethune International Peace Hospital, Department of Pharmacology, Hebei Medical University, Shijiazhuang, China.
Aim:
To investigate the stereoselectivity in absorption of trans tramadol (trans T) in rat intestine.
Methods:
The duodenum, jejunum and ileum were separately perfusated in situ with trans T dissolved in Krebs-Ringer buffer. Trans T enantiomers in the perfusate were analyzed with a high performance capillary electrophoresis (HPCE) method.
Results:
The absorbed fractions of trans T enantiomers were similar among the different segments of the rat intestine. The absorbed fraction of (+)-trans T was lower than that of (-)-trans T when the concentration of trans T was not higher than 40 mumol.L-1. As the concentration of trans T increased, the absorbed fractions of trans T enantiomers were reduced and the difference in absorbed fractions between trans T enantiomers became not significant.
Conclusion:
Trans T enantiomers can be absorbed in different parts of the rat intestine. The intestinal absorption of trans T was stereoselective, (-)-trans T being preferentially absorbed.
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