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[Study on the expression of the metastasis suppressor gene KAI1 in laryngeal squamous cell carcinoma]
Zu-liang Wu1, Zheng-hu Wu, Xing Guo
1Department of Otorhinolaryngology, First Affiliated Hospital, China Medical University, Shenyang 110001, China. entwzl@sina.com.cn
Objective:
To investigate the expression of the metastasis suppressor gene KAI1 in laryngeal squamous cell carcinoma and the relationship between expression of KAI1 and it's role in tumorgenesis and development.
Methods:
The sections of 84 samples of primary laryngeal squamous cell carcinoma (PLSCC), 27 samples of laryngeal precancerous lesion (LPL), 10 samples of vocal cords polyp (VCP) and 10 samples of normal laryngeal tissues (NLT) were examined by in situ hybridization for KAI1mRNA.
Results:
It was found that 10 samples of NLT and 10 samples of VCP showed strong expression of KAI1, the Integrated A Total of KIA1 in these two types of tissues were 136,206.8 +/- 36,675.536 and 133,674.505 +/- 42,858.456, respectively, with no statistically significant difference between them, and high prevalence of decreased expression of KAI1 in LPL and PLSCC compared with that in VCP and in NLT (P < 0.01). There was a significant relationship between down-regulation of KAI1 expression with pathological grade, lymph node metastases, T stage and clinical stage (P < 0.01) and did not correlate with gender.
Conclusion:
KAI1 may play an important role on tumor genesis, development, invasion and metastasis of PLSCC, and become one of the molecular markers for early diagnosis and predicting of aggressive and metastasis potential to assess the clinical stage of PLSCC.
