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[Effect of erythromycin on the expression of apoptotic gene on eosinophils in nasal polyps in vitro]
1Department of Otolaryngology, Head and Neck Surgery, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China.
Objective:
To study the expression of apoptotic gene in nasal polyps and the possible effect of erythromycin on it.
Methods:
Total 40 nasal polyps biopsies were detected the expression of Bcl-2, Bax, Bcl-xL as well as Fas protein on eosinophils by immunohistochemistry. The expression of Bax, Bcl-xL, Fas on eosinophils in 15 nasal polyps explants which cultured with or without erythromycin were detected also.
Results:
In nasal polyps, the expression of Bcl-2, Bax, Bcl-xL and Fas protein on eosinophils are 5% (2/10), 65% (26/40), 40% (16/40) and 90% (36/40) respectively. As compared with inferior turbinate, there were significant difference in expression of Bax and Fas protein between two tissues (P < 0.05). After cultured with erythromycin, the expression of Bax protein increased (P < 0.05), but the expression of Bcl-xL and Fas protein had not changed.
Conclusion:
Bax may be the most significant gene which control the apoptosis of eosinophils in nasal polyps. And a possible mechaism which erythromycin promote the apoptosis of eosinophils is increase the expression of Bax.
