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[Cloning and sequence analysis of the preprotein cDNA of human bone morphogenetic protein 12]
Yan-meng Lu1, Wen-yu Fu, Ying-jie Piao
1Central Laboratory, First Military Medical University, Guangzhou 510515, China. tse@fimmu.com
Objective:
To clone the cDNA of the preprotein cDNA of human bone morphogenetic protein 12 (hBMP12).
Methods:
Two primers were designed according to hBMP12 sequence reported in GenBank. The hBMP12 preprotein cDNA was obtained by reverse transcriptional (RT)-PCR from the mRNA extracted from human placenta, followed by cloning into pTARGETTM plasmid and sequence analysis of the plasmid pT(ARGE)T(TM)-BMP12.
Results:
DNA agarose gel electrophoresis showed that the product of RT-PCR was about 920 bp, as was consistent with the result of PCR detection of the recombinant plasmid. The result of sequence analysis was in agreement with the reported hBMP12 sequence in GenBank.
Conclusion:
The preprotein of hBMP12 cDNA has been successfully cloned with correct sequence.
