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Updated: Aug 25, 2026
TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
Published on: June 8, 2020
[Analysis of the difference in NIH3T3 cell protein expression profiles before and after TPA treatment using
Xin-ming Shen1, Pei-zhou Jiang, Hua Huang
1Institute of Cancer Research, First Military Medical University, Guangzhou 510515, China.
Objective:
To establish and optimize two-dimensional (2-D) polyacrylamide gel electrophoresis (PAGE) for comparative analysis of the protein expression profiles in mouse fibroblast NIH3T3 cells before and after 12-O-tetradecanoy- lphorbol-13-acetate (TPA) treatment using image analysis software, so as to prepare for more intensive study in the identification of the proteins that mediate the biological effect of TPA.
Methods:
The total cellular protein extracted from NIH3T3 cells with or without TPA treatment underwent 2-D PAGE and silver nitrate staining prior to the analysis of the differential protein expressions using image analysis software.
Results:
Image analysis revealed obvious differential protein expressions of the cells in response to TPA treatment.
Conclusion:
High-quality instruments for 2-D PAGE, skilled electrophoretic operation and efficient image processing are all essential in the reliable identification of stable functional proteins.
