Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A computational model for the electrostatic sequestration of PI(4,5)P2 by membrane-adsorbed basic peptides.

Jiyao Wang1, Alok Gambhir, Stuart McLaughlin

  • 1Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, New York 10021, USA.

Biophysical Journal
|March 26, 2004
PubMed
Summary

Basic peptides can sequester phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) in membranes through electrostatic interactions. Increased peptide charge density and decreased salt concentration enhance this PI(4,5)P2 sequestration.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

STNAGNN: Data-driven Spatio-temporal Brain Connectivity beyond FC.

Proceedings of machine learning research·2026
Same author

<i>MFAP2</i> Promotes Glioblastoma Malignant Phenotypes via Autophagy-Dependent Activation of Wnt/β-Catenin Signaling.

Biomedicines·2026
Same author

HYPERBOLIC MODEL AGGREGATION FOR FEDERATED LEARNING IN FMRI.

Proceedings. IEEE International Symposium on Biomedical Imaging·2026
Same author

Centromere protein I promotes hepatocellular carcinoma progression by activating PI3K/AKT/mTOR-CDK2 cascade.

Cancer biology & therapy·2026
Same author

Chlorzoxazone-based One-Sample Method for Estimating In Vivo CYP2E1 Activity in Mice.

Current drug metabolism·2026
Same author

Impact of POR and cytochrome b5 variability on the genotype-phenotype discordance of CYP2A6 and CYP2D6: A quantitative analysis in Chinese human livers.

Drug metabolism and disposition: the biological fate of chemicals·2026

Area of Science:

  • Membrane biophysics
  • Computational biophysics
  • Molecular interactions

Background:

  • Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) is a vital acidic phospholipid involved in numerous cellular functions.
  • Peripheral proteins with basic residue clusters can bind and sequester PI(4,5)P2 in cell membranes.
  • The myristoylated alanine-rich C kinase substrate (MARCKS) effector domain is known to interact with PI(4,5)P2.

Purpose of the Study:

  • To computationally investigate the electrostatic basis of PI(4,5)P2 sequestration by membrane-adsorbed basic peptides.
  • To quantitatively assess the factors influencing the binding affinity between basic peptides and PI(4,5)P2.

Main Methods:

  • Finite difference Poisson-Boltzmann calculations were employed.
  • Electrostatic free energy of PI(4,5)P2 sequestration was computed for various peptides (Lys-7, Lys-13, FA-MARCKS(151-175)).

Related Experiment Videos

  • The influence of peptide charge density, membrane lipid composition, and ionic strength was analyzed.

    • Main Results:

    • Calculations confirmed that basic peptides like Lys-13 and FA-MARCKS(151-175) can sequester multiple PI(4,5)P2 molecules.
    • Favorable sequestration occurred with increased peptide linear charge density and decreased membrane acidic lipid content.
    • Lowering solution ionic strength significantly enhanced the electrostatic free energy of sequestration.
    • The calculated electrostatic binding energy exceeded the entropic cost of localizing PI(4,5)P2.

    Conclusions:

    • Electrostatic interactions are the primary driving force for PI(4,5)P2 sequestration by basic peptide sequences.
    • The degree of PI(4,5)P2 sequestration is tunable by peptide charge and environmental conditions (ionic strength, lipid composition).
    • This study provides a quantitative framework for understanding how basic peptide domains interact with and regulate PI(4,5)P2 levels at membranes.