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Updated: Aug 25, 2026

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Rapid method for evaluating reversed-phase high-performance liquid chromatography column stability
Dania A Fonseca1, Humberto R Gutiérrez, Kenneth E Collins
1Instituto de Química, Universidade Estadual de Campinas, Caixa Postal 6154, 13084-971 Campinas, SP, Brazil.
Abstract:
A procedure is presented for the rapid evaluation of HPLC stationary phase stability at pH 8.4 or 10.1 using a temperature of 60 degrees C. Mobile phase (MeOH-0.1 mol l(-1) aqueous NaHCO3, 50:50, v/v) is continuously passed through the column with periodic injections of a test solution until the several chromatographic parameters of the resulting chromatograms are degraded. The tests were applied to several commercial and laboratory-made stationary phases. After degradation two of these phases, one commercial and one laboratory-made, were examined by elemental analysis and scanning electron microscopy to elucidate the degradation process.
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