Microwave-immobilized polybutadiene stationary phase for reversed-phase high-performance liquid chromatography
Nilva P Lopes1, Kenneth E Collins, Isabel C S F Jardim
1Laboratório de Pesquisas em Cromatografia Líquida, LABCROM, Instituto de Química, Universidade Estadual de Campinas, Caixa Postal 6154, CEP 13084-971 Campinas, SP, Brazil.
Abstract:
Polybutadiene (PBD) has been immobilized on high-performance liquid chromatography (HPLC) silica by microwave radiation at various power levels (52-663 W) and actuation times (3-60 min). Columns prepared from these reversed-phase HPLC materials, as well as from similar non-irradiated materials, were tested with standard sample mixtures and characterized by elemental analysis (%C) and infrared spectroscopy. A microwave irradiation of 20 min at 663 W gives a layer of immobilized PBD that presented good performance. Longer irradiation times give thicker immobilized layers having less favorable chromatographic properties.
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