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Updated: Aug 25, 2026
Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells
Published on: August 9, 2019
Peripheral blood circulating immature cell counts predict CD34+ cell yields in G-CSF-induced PBPC mobilization in
Teruhiko Kozuka1, Kazuma Ikeda, Takanori Teshima
1Second Department of Internal Medicine, Division of Blood Transfusion, Okayama University Medical School, Japan. c-tkozuka@eph.pref.ehime.jp
Insights
Circulating immature cell counts in peripheral blood can estimate CD34+ cell yield during stem cell collection. A threshold of 1.7 x 10^9 CICs/L predicts successful peripheral blood progenitor cell collections.
Area of Science:
- Hematology
- Cellular Biology
- Transplantation Science
Background:
- Circulating immature cells (CIC) in peripheral blood (PB) may estimate CD34+ cell yield during G-CSF plus chemotherapy-induced PBPC mobilization.
- Previous studies suggest a correlation, but its clinical utility requires further evaluation.
Purpose of the Study:
- To evaluate the correlation between CIC counts in PB and CD34+ cell yield in G-CSF-induced PBPC mobilization for healthy donors.
- To determine the usefulness of CIC counts as a predictor for successful apheresis collections.
Main Methods:
- Assessed CIC counts in PB and CD34+ cell counts in apheresis products from 122 collections.
- Utilized Pearson rank correlation analysis, chi-squared test, and U-test to evaluate the relationship between CIC and CD34+ cell counts.
Main Results:
- A weak correlation was found between CIC counts and CD34+ cells/L in the apheresis product (r=0.357, p<0.0001).
- A cutoff CIC count of 1.7 x 10^9/L demonstrated 63.6% sensitivity and 77.5% specificity for predicting >20 x 10^6 CD34+ cells/L collection.
Conclusions:
- CIC counts in PB can serve as an estimate for collected CD34+ cells.
- CIC counts exceeding 1.7 x 10^9/L are a reliable predictor for achieving >20 x 10^6 CD34+ cells/L in a single apheresis procedure.
Background:
It has been previously reported that the number of circulating immature cells (CIC) in peripheral blood (PB) estimates the number of CD34+ cells collected in G-CSF plus chemotherapy-induced PBPC mobilization. The correlation of CIC counts in PB with CD34+ cell yield and its usefulness was evaluated in G-CSF-induced PBPC mobilization for healthy donors.
Study Design And Methods:
CIC counts in PB and CD34+ cell counts in the apheresis product from 122 collections were assessed, and the relationship between these two variables was evaluated with the Pearson rank correlation analysis, the chi-squared test, and the U-test.
Results:
CIC counts were correlated weakly with the number of CD34+ cells per L of blood processed in the apheresis product (Pearson rank correlation analysis; r=0.357, p<0.0001). When a level of 1.7 x 10(9) CICs per L was selected as a cutoff value, the sensitivity and specificity for collecting more than 20 x 10(6) CD34+ cells per L of blood processed were 63.6 and 77.5 percent, respectively.
Conclusion:
The present study suggests that the number of CICs in PB may estimate the number of CD34+ cells collected. The data indicate that CIC counts above 1.7 x 10(9) per L can be used as a good predictor for PBPC collections containing more than 20 x 10(6) CD34+ cells per L of blood processed in a single apheresis procedure.
