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Related Experiment Videos

Complement activation in prestorage leucocyte-filtered plasma.

M Hyllner1, M Tylman, J P Bengtson

  • 1Department of Anaesthesiology & Intensive Care, Sahlgrenska University Hospital, Goteborg University, Goteborg, Sweden. monica.hyllner@vgregion.se

Transfusion Medicine (Oxford, England)
|March 27, 2004
PubMed
Summary

Prestorage leucocyte filtration of plasma activates complement but does not reduce inflammatory cytokine generation during blood product storage. Complement activation occurs in all stored blood products, regardless of filtration.

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Area of Science:

  • Transfusion Medicine
  • Immunology
  • Biochemistry

Background:

  • Blood component storage can lead to complement activation and pro-inflammatory cytokine release.
  • Prestorage leucocyte filtration of blood components reduces cytokine accumulation but does not eliminate transfusion reactions.

Purpose of the Study:

  • To investigate inflammatory mediator release during storage of whole blood and plasma.
  • To assess the impact of prestorage leucocyte filtration on plasma during storage.

Main Methods:

  • Collected 24 blood units (whole blood, plasma, filtered plasma) and stored for 35 days.
  • Weekly analysis of potassium, leucocytes, free plasma haemoglobin, complement activation (C3a, SC5b-9), and cytokines (IL-6, IL-8, TNF-alpha).

Main Results:

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  • Elevated C3a and SC5b-9 in filtered plasma from storage initiation; C3a levels increased over time.
  • Higher rate of change in C3a and SC5b-9 during storage in plasma compared to filtered plasma.
  • Interleukin-8 released in whole blood; low cytokine levels in plasma and filtered plasma.

Conclusions:

  • Complement activation occurs in whole blood, plasma, and filtered plasma during storage.
  • Prestorage filtration of plasma activates the complement cascade.
  • Filtration does not influence cytokine generation in stored plasma.