Jove
Visualize
Contact Us

Related Experiment Videos

Oxygen sensing and oxidant/redox-related pathways.

John J Haddad1

  • 1Severinghaus-Radiometer Research Laboratories, University of California, San Francisco, CA, USA. johnjhaddad@yahoo.co.uk

Biochemical and Biophysical Research Communications
|March 27, 2004
PubMed
Summary

Organisms sense oxygen variations through changes in oxidative stress and redox homeostasis, impacting gene transcription. Transcription factors like HIF-1alpha and NF-kappaB may act as key oxygen sensors.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hypoxia upregulates MAPK(p38)/MAPK(ERK) phosphorylation in vitro: neuroimmunological differential time-dependent expression of MAPKs.

Protein and peptide letters·2014
Same author

The immunopharmacologic potential of Semaxanib and new generation directed therapeutic drugs: Receptor tyrosine kinase regulation with anti-tumorigenensis/angiogenesis properties.

Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society·2013
Same author

The bioanalytical molecular pharmacology of the N-methyl-D-aspartate (NMDA) receptor nexus and the oxygen-responsive transcription factor HIF-1α: putative mechanisms and regulatory pathways unravel the intimate hypoxia connection.

Current molecular pharmacology·2013
Same author

Current opinion on 3-[2-[(2-tert-butyl-phenylaminooxalyl)-amino]-propionylamino]- 4-oxo-5-(2,3,5,6-tetrafluoro-phenoxy)-pentanoic acid, an investigational drug targeting caspases and caspase-like proteases: the clinical trials in sight and recent anti-inflammatory advances.

Recent patents on inflammation & allergy drug discovery·2013
Same author

Measurement of antioxidant activity and antioxidant compounds under versatile extraction conditions: II. The immuno-biochemical antioxidant properties of black sour cherry (Prunus cerasus) extracts.

Anti-inflammatory & anti-allergy agents in medicinal chemistry·2013
Same author

Measurement of antioxidant activity and antioxidant compounds under versatile extraction conditions: I. the immuno-biochemical antioxidant properties of sweet cherry (Prunus avium) extracts.

Anti-inflammatory & anti-allergy agents in medicinal chemistry·2013
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Cellular Biology
  • Molecular Biology
  • Physiology

Background:

  • Altered reduction-oxidation (redox) homeostasis and oxidative stress are key regulators of gene transcription.
  • Dynamic changes in oxygen homeostasis are closely linked to cellular redox equilibrium.

Purpose of the Study:

  • To investigate the nature of oxygen sensors in organisms.
  • To elucidate how organisms sense variations in oxygen levels.
  • To explore the role of transcription factors in oxygen sensing.

Main Methods:

  • Analysis of gene transcription regulation.
  • Investigation of cellular redox state and homeostasis.
  • Examination of signaling pathways involving HIF-1alpha and NF-kappaB.

Related Experiment Videos

Main Results:

  • Progressive oxidative stress linked to altered redox homeostasis regulates gene transcription.
  • Oxygen homeostasis changes act as signaling mechanisms for oxygen-sensing pathways.
  • Oxygen-evoked regulation of HIF-1alpha and NF-kappaB is coupled with intracellular redox state.

Conclusions:

  • Transcription factors, including HIF-1alpha and NF-kappaB, are potential oxygen sensors.
  • Intracellular redox machinery plays a crucial role in regulating oxygen- and redox-sensitive transcription factors.
  • Understanding these pathways is vital for deciphering oxygen sensing mechanisms.