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Functional Complementation Analysis (FCA): A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways
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How good are quantitative complementation tests?

Philip M Service1

  • 1Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011, USA. Philip.Service@nau.edu

Science of Aging Knowledge Environment : SAGE KE
|March 27, 2004
PubMed

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Complementation Tests00:49

Complementation Tests

A complementation test is a simple cross to identify whether the two mutations are located on the same gene or different genes. It was first performed by Edward Lewis in the 1940s while working on fruit flies. He developed the test to identify the location and arrangement of different mutations on chromosomes.
Organisms heterozygous for different mutations are crossed pairwise in all combinations. If present on different genes, the mutations can complement each other by providing the missing...