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Related Concept Videos

Protein Organization01:13

Protein Organization

Overview
Protein Organization01:13

Protein Organization

Overview
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Protein Organization01:24

Protein Organization

Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence.
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Protein Organization01:24

Protein Organization

Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence.

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Related Experiment Video

Updated: Jul 18, 2026

The MultiBac Protein Complex Production Platform at the EMBL
13:51

The MultiBac Protein Complex Production Platform at the EMBL

Published on: July 11, 2013

Structure-based assembly of protein complexes in yeast.

Patrick Aloy1, Bettina Böttcher, Hugo Ceulemans

  • 1European Molecular Biology Laboratory, Structural and Computational Biology Programme, 1, 69117 Heidelberg, Germany.

Science (New York, N.Y.)
|March 27, 2004
PubMed
Summary

This study models yeast protein complexes using structural interactions and electron microscopy, revealing atomic details for 29 complexes and mapping cellular molecular machine networks.

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Area of Science:

  • Molecular biology
  • Structural biology
  • Systems biology

Background:

  • Understanding cellular molecular machines requires bridging the gap between whole-cell images and atomic structures.
  • Protein-protein interactions, bioinformatics, and electron microscopy are key tools for this endeavor.

Purpose of the Study:

  • To model yeast protein complexes using known 3D structures and protein interactions.
  • To determine atomic details of complexes not easily resolved by homology modeling alone.
  • To construct a network of interacting molecular machines within the cell.

Main Methods:

  • Utilizing known three-dimensional protein structures to model yeast complexes.
  • Employing electron microscopy for screening and validation of modeled complexes.
  • Analyzing inter-complex interactions (cross-talk) to build a network.

Main Results:

  • Successfully obtained at least partial models for 54 out of 102 yeast complexes.
  • Resolved atomic details for 29 complexes, including the exosome, chaperonin containing TCP-1, 3'-messenger RNA degradation complex, and RNA polymerase II.
  • Identified novel structural insights through the combination of multiple known structures.

Conclusions:

  • The integration of structural data and electron microscopy enables detailed modeling of cellular machinery.
  • This approach provides atomic-level insights into complex biological processes and inter-complex relationships.
  • A structure-based network of molecular machines offers a systems-level view of cellular organization and function.