Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A monomeric myosin VI with a large working stroke.

Ida Lister1, Stephan Schmitz, Matthew Walker

  • 1MRC Laboratory of Molecular Biology, Hills Road, Cambridge, UK.

The EMBO Journal
|March 27, 2004
PubMed
Summary

Myosin VI, a unique motor protein, moves towards actin filament minus ends. It exhibits nonprocessive movement with an 18 nm working stroke, challenging traditional myosin models.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Benchmarking DNA extraction protocols across use cases for culture-independent Nanopore metagenomics.

Microbial genomics·2026
Same author

Cord-blood black carbon particle burden is associated with a C19MC small extracellular vesicle miRNA signature enriched for neurodevelopmental pathways.

Environmental research·2026
Same author

A sensitive method for the determination of novel derivatised thyrostat residues in urine by using UHPLC-MS/MS.

Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment·2026
Same author

Cord-blood black carbon burden is associated with coordinated inflammatory and heme-metabolic transcriptional programs at birth in Bradford, United Kingdom.

The Science of the total environment·2026
Same author

Plasmodium falciparum PfMyoF contains a Rab-like tail domain and associates with perinuclear membrane trafficking proteins.

Communications biology·2026
Same author

Erratum: Wide-field and non-invasive imaging of brain tumours with scattered light techniques: erratum.

Biomedical optics express·2026

Area of Science:

  • Cell Biology
  • Molecular Motors
  • Biophysics

Background:

  • Myosin VI plays roles in endocytosis, secretion, and cell migration.
  • Its unique minus-end directed movement suggests distinct cellular functions.
  • The mechanism of myosin VI force production and movement remains unclear.

Purpose of the Study:

  • To investigate the mechanism of force production and movement in myosin VI.
  • To understand the unique properties of myosin VI related to its cellular functions.

Main Methods:

  • Utilized electron microscopy with negative staining to observe conformational changes.
  • Employed an optical tweezers-based force transducer to measure mechanical properties.
  • Studied both expressed full-length and native monomeric myosin VI.

Related Experiment Videos

Main Results:

  • ATP addition induced a significant conformational change in the neck/tail region of myosin VI.
  • Myosin VI demonstrated nonprocessive movement with a large 18 nm working stroke.
  • The short neck region of myosin VI challenges the classical lever arm mechanism.

Conclusions:

  • Myosin VI's large working stroke may involve contributions from structures beyond the neck region.
  • A novel model is proposed to explain the 18 nm working stroke of myosin VI.
  • Further research is needed to fully elucidate myosin VI's unique mechanical properties and cellular roles.