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Updated: Aug 25, 2026

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Automated high-performance liquid chromatographic analysis and post-column photoderivatization of Iotetrol(R) in
Abstract:
An automated procedure for sample work-up and preparation of calibration standards for HPLC analyses is presented. The equipment consisting of a benchtop workstation and a conventional HPLC system was used to analyze aqueous solutions containing Iotetrol, a contrast medium. The four rotamers of the test compound were separated using the stationary phase Hypercarb S and by elution with tetrahydro-furan/water 3:97 (w/w). The analytical procedure was characterized with respect to sensitivity, selectivity, linearity of the detector response, accuracy, precision and carry-over effect. The use of the workstation yielded excellent data with respect to accuracy (102%) and precision (1%). At the stage of method development, the influence of post-column photoderivatization on the detectability of the analyte was investigated. As a result, a bathochromic shift of the UV absorbance maximum and the considerable intensification of the rudimentary fluorescence properties of the Iotetrol molecule were observed. The automatization saved a considerable amount of man-hours (ca. 25%).
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