Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

ATP overflow from the mouse isolated vas deferens.

M E Drake1, S A Petersen

  • 1Department of Physiology, Leicester University.

British Journal of Pharmacology
|April 1, 1992
PubMed
Summary

Field stimulation of mouse vas deferens releases significant adenosine 5'-triphosphate (ATP). ATP overflow increases with stimulus number and frequency, indicating pre-junctional release.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antemortem diagnosis of localized central nervous system histiocytic sarcoma in 2 dogs.

Journal of veterinary internal medicine·2009
Same author

New opportunities for nurses in medical education: facilitating valuable community learning experiences.

Nurse education in practice·2008
Same author

Canine intraspinal meningiomas: imaging features, histopathologic classification, and long-term outcome in 34 dogs.

Journal of veterinary internal medicine·2008
Same author

Distinguishing between natural and aquaculture-derived sediment concentrations of heavy metals in the Broughton Archipelago, British Columbia.

Marine pollution bulletin·2007
Same author

The use of meiofauna as an indicator of benthic organic enrichment associated with salmonid aquaculture.

Marine pollution bulletin·2007
Same author

Effect of parental smoking on cotinine levels in newborns.

Archives of disease in childhood. Fetal and neonatal edition·2007

Area of Science:

  • Neuroscience
  • Pharmacology
  • Physiology

Background:

  • Adenosine 5 '-triphosphate (ATP) is a key neurotransmitter in the autonomic nervous system.
  • Measuring ATP release in response to physiological stimuli is crucial for understanding its role.

Purpose of the Study:

  • To quantify adenosine 5 '-triphosphate (ATP) overflow from field-stimulated mouse vas deferens in vitro.
  • To investigate the relationship between stimulation parameters and ATP release.

Main Methods:

  • Utilized a modified luciferin-luciferase assay for sensitive ATP detection.
  • Employed precise timing for stimulation, superfusate collection, and assay to minimize ATP degradation.
  • Stimulated mouse vas deferens with varying numbers and frequencies of pulses.

Main Results:

  • Field stimulation (50-200 pulses at 5 Hz) increased ATP overflow 7-28 fold over basal levels.
  • ATP overflow showed a dose-dependent increase with the number of stimuli.
  • Elevated stimulation frequency above 10 Hz led to increased ATP overflow, suggesting a balance between release and degradation.
  • Exogenous phenylephrine-induced contractions caused minimal ATP overflow, indicating a predominantly pre-junctional origin of stimulation-induced ATP release.

Conclusions:

  • Field stimulation is a potent method for releasing ATP from the mouse vas deferens.
  • ATP release is influenced by both the number and frequency of stimuli.
  • The majority of stimulation-induced ATP overflow appears to originate from pre-junctional sites.

Related Experiment Videos