[Flow cytometric study of low density lipoprotein receptors: biological and clinical interest]

Low density lipoprotein (LDL) carry 60 to 80% of plasma cholesterol. These particules are mainly catabolized by LDL receptor. Thus, LDL receptor plays an important role in the regulation of plasma LDL cholesterol concentration, which is correlated to the risk of developing cardiovascular disease. The intracellular concentration of free cholesterol exerts a negative feedback on LDL receptor expression at the cell surface. This expression is also modulated by a lot of hormones, drugs and cytokines. On the other hand, mutations of LDL receptor induce familial hypercholesterolaemia, a frequent genetic disorder (1 to 500 births for the heterozygous form). Flow cytometry is a simple and fast technic, allowing to quantify LDL receptor expression at the cell surface and to study its functionality. Two different ligands can be used with flow cytometry: either fluorochrome-labelled LDL or anti-LDL receptor monoclonal antibody detected by a fluorescent secondary antibody. In immunofluorescent assays, we can now precisely calculate the number of LDL receptor at the cell surface by using calibration kits. In this article, we summarize the different regulatory factors of LDL receptor expression and present the advantages and limits of the different flow cytometry assays for LDL receptor analysis.