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Quantitative determination of transferrin in cerebrospinal fluid using an enzyme linked immunosorbent assay

G F Van Landeghem1, P C D'Haese, L V Lamberts

  • 1University of Antwerp, Department of Nephrology-Hypertension, University Hospital Antwerp, Wilrijkstraat 10, B-2650, Edegem/Antwerpen, Belgium.

Analytical and Bioanalytical Chemistry
|April 1, 1996
PubMed

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.