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Published on: April 20, 2018
[The interaction of stilbazolium merocyanines with hyperproliferative laryngeal papilloma cells--preliminary study]
Jarosław Szydłowski1, Justyna Cofta, Krzysztof Wiktorowicz
1Klinika Otolaryngologii Dzieciecej Instytutu Pediatrii AM im. Karola Marcinkowskiego w Poznaniu.
Abstract:
Recently increasing interest focused in possible clinical application on photodynamic therapy was observed. Many scientists are looking for new chemical compounds effective in therapy and giving no side effects. Stilbazolium merocyanines belong to lipophilic dyes, binding to cell membranes. The aim of this study was to estimate the usefulness of the selected stilbazolium merocyanines to treatment on human recurrent respiratory papillomatosis. A patients group consisted of seven children, ages 10 months to 18 years, with moderate to severe recurrent disease. The patients were treated surgically. Tissue samples were analyzed for presence of Human papillomavirus (HPV). The incorporation of the dye into papillomas cells membranes was measured by flow cytometry. As a measure of fluorescence intensitivity the value "mean channel" was used. The hyperplastic tissue showed the highest fluorescence intensity than normal epithelium. Obtained results showed that continue to this preliminary studies is necessary.
Insights
Stilbazolium merocyanines show potential for treating recurrent respiratory papillomatosis by targeting papilloma cell membranes. Further research is needed to confirm their clinical effectiveness and safety.
Area of Science:
- Photodynamic therapy
- Oncology
- Dermatology
Background:
- Photodynamic therapy (PDT) is gaining interest for clinical applications.
- Researchers seek novel, safe therapeutic compounds.
- Stilbazolium merocyanines are lipophilic dyes that bind to cell membranes.
Purpose of the Study:
- To evaluate stilbazolium merocyanines for treating human recurrent respiratory papillomatosis (RRP).
- To assess the dye's incorporation into papilloma cell membranes.
Main Methods:
- Seven pediatric patients with moderate to severe RRP were studied.
- Human papillomavirus (HPV) presence was analyzed in tissue samples.
- Flow cytometry measured dye incorporation into cell membranes, using "mean channel" for fluorescence intensity.
Main Results:
- Hyperplastic papilloma tissue exhibited higher fluorescence intensity compared to normal epithelium.
- The study demonstrated the feasibility of measuring dye uptake in RRP tissues.
Conclusions:
- Stilbazolium merocyanines show preliminary promise for RRP treatment.
- Further studies are necessary to validate these findings and explore clinical utility.
