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Updated: Aug 16, 2026

Phase Contrast and Differential Interference Contrast (DIC) Microscopy
Published on: August 6, 2008
Linear phase imaging using differential interference contrast microscopy
M R Arnison1, K G Larkin, C J R Sheppard
1Physical Optics Laboratory, School of Physics, University of Sydney, NSW 2006, Australia. mra@cisra.canon.com.au
Abstract:
We propose an extension to Nomarski differential interference contrast microscopy that enables isotropic linear phase imaging. The method combines phase shifting, two directions of shear and Fourier-space integration using a modified spiral phase transform. We simulated the method using a phantom object with spatially varying amplitude and phase. Simulated results show good agreement between the final phase image and the object phase, and demonstrate resistance to imaging noise.
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