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Published on: February 24, 2016
Tissue-engineered buccal mucosa for substitution urethroplasty
S Bhargava1, C R Chapple, A J Bullock
1Department of Urology, Section of Reconstruction, Urodynamics and Female Urology, Royal Hallamshire Hospital, Glossop Road, Sheffield S10 2JF, South Yorkshire, UK.
BJU International
|March 31, 2004
Summary
Tissue-engineered buccal mucosa (TEBM) was successfully developed for urethral reconstruction. This robust TEBM is suitable for clinical substitution urethroplasty, addressing limitations in current grafting tissues.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Urethral reconstruction faces limitations due to graft tissue availability.
- Buccal mucosa (BM) is increasingly favored for urethral substitution.
- Developing robust engineered tissues is crucial for reconstructive surgery.
Purpose of the Study:
- To engineer buccal mucosa (TEBM) for substitution urethroplasty.
- To create a reliable tissue substitute for urethral reconstruction.
- To overcome limitations of autologous tissue grafts.
Main Methods:
- Enzymatic and mechanical separation of buccal mucosa biopsy.
- Isolation and expansion of oral keratinocytes and fibroblasts.
- Seeding cells onto de-epidermized dermis (DED) to create full-thickness TEBM.
- Assessing keratinocyte migration with MTT assay and histological analysis post-mechanical stress.
Main Results:
- Cultured TEBM histologically resembled native oral mucosa after 2 weeks.
- Demonstrated good horizontal keratinocyte migration on DED.
- Showed epidermal thickening and dermal remodeling over 14 days.
- Maintained epidermal-dermal junction integrity under mechanical stress.
Conclusions:
- Successfully cultured full-thickness TEBM for substitution urethroplasty.
- Developed TEBM is robust and suitable for clinical application.
- This engineered tissue offers a promising solution for urethral reconstruction.
