Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Increase in intranuclear nuclear factor kappaB and decrease in inhibitor kappaB in mononuclear cells after a mixed

Ahmad Aljada1, Priya Mohanty, Husam Ghanim

  • 1Division of Endocrinology, Diabetes and Metabolism, State University of New York at Buffalo and Kaleida Health, Buffalo, NY, USA.

The American Journal of Clinical Nutrition
|March 31, 2004
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Ageing Adipose Paradox: Implications for Metabolic Health.

Diabetes, obesity & metabolism·2026
Same author

<i>Chlamydi</i><i>a pneumoniae</i> Seropositivity and Acute Coronary Syndromes: A Case-Control Study of the Infectious-Inflammatory Axis.

Medicina (Kaunas, Lithuania)·2026
Same author

Vitamin D status and <i>Helicobacter pylori</i> infection: clinical associations and lipid pathway differences in an exploratory metabolomics sub-study.

Frontiers in nutrition·2026
Same author

Mass spectrometry-based proteomics of FFPE tissues: progress, limitations, and clinical translation barriers.

Clinical proteomics·2025
Same author

Unlocking the secrets: Adipose tissue dysfunction and atherosclerosis-mechanisms and innovative therapeutic approaches.

Atherosclerosis·2025
Same author

Adipose tissue dysfunction disrupts metabolic homeostasis: mechanisms linking fat dysregulation to disease.

Frontiers in endocrinology·2025
This summary is machine-generated.

A mixed meal significantly increases reactive oxygen species (ROS) generation and inflammatory markers in leukocytes. These findings suggest a link between meal intake and chronic inflammatory conditions like obesity and atherosclerosis.

Area of Science:

  • Biochemistry
  • Immunology
  • Nutrition Science

Background:

  • Glucose intake stimulates reactive oxygen species (ROS) generation.
  • Investigating the link between mixed meals, ROS production, and inflammation is crucial.

Purpose of the Study:

  • To determine if a 900-kcal mixed meal increases ROS generation by leukocytes.
  • To assess if a mixed meal induces cellular-level inflammatory responses.

Main Methods:

  • Nine subjects consumed a 900-kcal mixed meal; eight consumed water.
  • Blood samples analyzed for ROS generation, p47(phox) expression, NF-kappaB activity, IkappaBalpha, IKKalpha, IKKbeta, CRP, and soluble intercellular adhesion molecule.
  • Measurements taken at 0, 1, 2, and 3 hours post-ingestion.

Related Experiment Videos

Main Results:

  • Mixed meal intake significantly increased ROS generation and p47(phox) expression in leukocytes.
  • NF-kappaB binding activity and IKKalpha/IKKbeta expression increased, while IkappaBalpha decreased.
  • Plasma C-reactive protein (CRP) levels rose significantly post-meal; water intake had no effect.

Conclusions:

  • Mixed meal consumption triggers significant inflammatory changes, including decreased IkappaBalpha and increased NF-kappaB binding, CRP, IKKalpha, IKKbeta, and p47(phox) expression.
  • These meal-induced inflammatory changes may contribute to chronic conditions like hypertension, obesity, and atherosclerosis.