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[Rat model for the multidrug resistant glioma cell line]
Jian Zhang1, Qing-lin Zhang, Cheng-wei Wang
1Department of Neurosurgery, Second Hospital of Shandong University, Jinan 250033, China.
Objective:
To evaluate the animal model of the multidrug resistant glioma cell line C6/adr for further in vivo studies.
Methods:
The rat glioma cells C6 and multidrug resistance cells C6/adr were cultured in vitro and implanted into the brain of S-D rats. After implantation, all these animals were examined continually with magnetic resonance imaging (MRI) and histological examination. The growth procedure of intracranial implanted glioma and the survival span of the animal model were evaluated. The statistical analysis was made between the survival data of the two cell lines.
Results:
The symptoms of intracranial hypertension did not occur until 4 weeks after inoculation. The MRI findings of the implanted glioma in the rat brain were much earlier than the abnormal behavior observed. Pathological results after inoculation demonstrated the MRI findings. The two cell lines had similar growth characteristics and no significant differences in survival times.
Conclusion:
These results suggest that by means of MRI and histology the growth procedure of the implanted glioma in vivo be successfully observed. All these data will proved to be a useful basis for study of glioma in vivo.