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Published on: January 16, 2021
Effect of process parameters on production of a biopolymer by Rhizobium sp
Flávia Pereira Duta1, Francisca Pessôa De França, Eliana Flávia Camporese Sérvulo
1Universidade Federal do Rio de Janeiro, Departamento de Engenharia Bioquímica, Bloco E, Ilha do Fundão, Rio de Janeiro, RJ CEP: 21949-900 Brazil.
Abstract:
The production of biopolymers by a Rhizobium strain was studied under batch and bioreactor conditions. The best viscosity levels were obtained under low mannitol concentrations as well as low agitation and aeration conditions. Infrared spectra indicated the presence of chemical groups characteristic of microbially produced biopolymers, including C = O and O-acetyl groups. Thermogravimetric analysis showed the characteristic degradation profiles of the exopolysaccharide produced (T(onset) = 290degreesC). The experimental design showed that a low substrate concentration (10.0 g/L), and low aeration (0.2 vvm) and agitation (200 rpm) levels should be used. The maximum yield of the process was a Yp/s (g/g) of 0.19 +/- 0.1, obtained under optimized conditions.
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