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Human HPRT mutant database: software for data entry and retrieval.

N F Cariello1, T R Craft, H Vrieling

  • 1Pathology Department, University of North Carolina, Chapel Hill 27599.

Environmental and Molecular Mutagenesis
|January 1, 1992
PubMed
Summary

A new database catalogs over 1,000 human hypoxanthine guanine phosphoribosyl transferase (HPRT) mutants, facilitating standardized data contribution and access for researchers studying HPRT mutations.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Bioinformatics

Background:

  • The human hypoxanthine guanine phosphoribosyl transferase (HPRT) gene is crucial for purine metabolism.
  • HPRT mutations are associated with Lesch-Nyhan syndrome and are widely used as a selectable marker in genetic studies.
  • A centralized, accessible repository for HPRT mutant data is needed to advance research.

Purpose of the Study:

  • To establish a comprehensive computer database of human hypoxanthine guanine phosphoribosyl transferase (HPRT) mutants.
  • To provide researchers with tools for standardized data input and efficient data retrieval.
  • To facilitate collaborative research and analysis of HPRT mutations.

Main Methods:

  • Development of a dBASE-formatted database containing over 1,000 published and unpublished HPRT mutant entries.

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  • Creation of a suite of user-friendly programs for data examination, extraction, and manipulation.
  • Implementation of a standardized data input program for ongoing contributions.
  • Distribution of the database and associated software via direct access or remote FTP.

    • Main Results:

    • A curated database with information on >1,000 human HPRT mutants is now available.
    • Software tools enable flexible data querying, including ordering by base pair position, filtering by mutagenic agent, and searching by author.
    • The system allows for the generation of customized reports, which can be printed or saved.
    • The database is designed for regular updates and redistribution.

    Conclusions:

    • This HPRT mutant database and associated software provide a valuable resource for the scientific community.
    • The standardized format and accessible tools promote efficient data sharing and analysis of HPRT mutations.
    • The resource will be regularly updated, ensuring its continued relevance and utility in genetic research.