Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Enteroviral hepatitis in children.

Hisashi Kawashima1, Shimizu Ryou, Shigeo Nishimata

  • 1Department of Pediatrics, Tokyo Medical University, Tokyo, Japan.

Pediatrics International : Official Journal of the Japan Pediatric Society
|April 2, 2004
PubMed
Summary

Enteroviruses were identified as the cause of hepatitis in over half of children with unknown illnesses. Reverse transcriptase-polymerase chain reaction (RT-PCR) of serum is an effective diagnostic method for enteroviral diseases.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efficacy of fenfluramine in a pediatric epilepsy patient with a pathogenic SV2A variant: A case report.

Epileptic disorders : international epilepsy journal with videotape·2026
Same author

Bowel Preparation Outcomes Using Low-Volume Polyethylene Glycol With Selective Rescue Enema in Pediatric Colonoscopy.

Pediatrics international : official journal of the Japan Pediatric Society·2026
Same author

Serum and CSF cytokine profile impact clinical phenotype in pediatric MOGAD.

Journal of neuroinflammation·2026
Same author

Secondary primary tumors following iodine-125 low-dose-rate brachytherapy for localized prostate cancer.

International journal of clinical oncology·2026
Same author

Characteristics of monogenic inflammatory bowel disease in very early-onset cases: a Japanese multicenter registry study.

Intestinal research·2026
Same author

Development of a zebrafish model of Loeys-Dietz syndrome through tgfbr2b knockdown.

Journal of human genetics·2026

Area of Science:

  • Virology
  • Hepatology
  • Pediatrics

Background:

  • Enteroviral hepatitis diagnosis is challenging due to difficulties in pathogen detection.
  • Cytomegalovirus (CMV) coinfection can complicate diagnosis.

Purpose of the Study:

  • To investigate the role of enteroviruses in pediatric hepatitis of unknown etiology.
  • To evaluate the utility of RT-PCR for enterovirus detection in serum and liver tissue.

Main Methods:

  • RT-PCR was used to detect enterovirus RNA in serum and liver samples.
  • Gene homology search identified specific enterovirus strains (Coxsackie B5/B6).
  • Liver pathology and viral load quantification were assessed.

Main Results:

Related Experiment Videos

  • Enterovirus RNA was detected in 9/21 serum samples (43%) and 3/3 liver tissue samples.
  • CMV-seropositive cases were negative for enterovirus.
  • Nine of 16 cases (56%) remained of unknown etiology after enterovirus testing.
  • Mild fibrosis and inflammation were observed in liver biopsies.
  • Viral load decreased during convalescence.

    • Conclusions:

    • Enteroviruses are a significant cause of pediatric hepatitis with unknown etiology.
    • RT-PCR is a valuable tool for diagnosing enteroviral infections.
    • Serum enterovirus quantification aids in disease identification.