Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Myosin V processivity: multiple kinetic pathways for head-to-head coordination.

Josh E Baker1, Elena B Krementsova, Guy G Kennedy

  • 1Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, VT 05405, USA.

Proceedings of the National Academy of Sciences of the United States of America
|April 2, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of UCB-9721 as a potent inhibitor of MyoA, the essential class XIV myosin motor of apicomplexan parasites.

bioRxiv : the preprint server for biology·2026
Same author

Molecular classification and association with survival outcomes in high-intermediate and high-risk early-stage endometrial cancers: Ancillary analysis of GOG-0249.

Gynecologic oncology·2026
Same author

Strength in Recovery: A Practice-Informed Commentary on Peer Recovery Specialists Facilitating Gambling Recovery Groups.

Journal of gambling studies·2026
Same author

Cryo-EM Structure of Human ATAD2B Reveals a Hexameric Organization Contributes to ATPase Activity and Substrate Coordination.

bioRxiv : the preprint server for biology·2026
Same author

Cargo Recognition of Nesprin-2 by the Dynein Adapter Bicaudal D2 for a Nuclear Positioning Pathway That Is Important for Brain Development.

Biochemistry·2026
Same author

Gynecologic oncology education for obstetrics and gynecology residents: a needs assessment.

Gynecologic oncology reports·2026

Myosin V molecular motors use a complex system of cooperative mechanisms, not just one, for head coordination. This ensures robust organelle transport within cells under various physiological conditions.

Area of Science:

  • Molecular biology
  • Cellular mechanics
  • Biophysics

Background:

  • Myosin V is a crucial double-headed molecular motor responsible for intracellular organelle transport.
  • Processive movement along actin filaments necessitates precise coordination between the two myosin heads.
  • Existing models propose single mechanisms for head coordination, which may be insufficient to explain robust motility.

Purpose of the Study:

  • To elucidate the underlying mechanisms of head coordination in Myosin V.
  • To investigate how nucleotide content influences the coordination and processivity of Myosin V.
  • To challenge and refine current understandings of molecular motor function.

Main Methods:

  • Single-molecule assays were employed to observe Myosin V processive runs.

Related Experiment Videos

  • Nucleotide content was systematically varied to perturb motor function.
  • Kinetic pathways were analyzed to understand head-head communication.

    • Main Results:

    • Myosin V head coordination is achieved through an elaborate system of cooperative mechanisms, not a single pathway.
    • Multiple kinetic pathways contribute to the communication between the two heads.
    • These cooperative mechanisms provide redundancy and ensure reliable motor function.

    Conclusions:

    • The coordination of Myosin V heads is more complex than previously thought, involving multiple synergistic mechanisms.
    • This intricate system guarantees robust processivity, allowing Myosin V to function effectively under diverse physiological demands.
    • Understanding these cooperative mechanisms offers insights into the general principles of molecular motor regulation.