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Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
Published on: October 16, 2017
Molecular characterization of a Rhodotorula-lytic enzyme from Paecilomyces lilacinus having beta-1,3-mannanase
Hiroyuki Sugino1, Shinya Furuichi, Sawao Murao
1Department of Applied Life Science, Faculty of Engineering, Sojo University. Ikeda, Kumamoto, Japan. hsugino@life.sojo-u.ac.jp
Abstract:
We cloned the gene and corresponding cDNA for an extracellular Rhodotorula-lytic enzyme which has beta-1,3-mannase activity, tentatively named MAN5C, from Paecilomyces lilacinus. MAN5C showed a high homology score with the members of glycoside hydrolase family 5 in a domain search with the Pfam database, indicating that MAN5C is a novel and unique member of glycoside hydrolase family 5.
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