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Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
Published on: June 12, 2018
Telomerase activity of peripheral blood mononuclear cells from patients with laryngeal carcinoma
PingJiang Ge1, BaoQuan Zhang, JianMin Zhang
1Department of Otolaryngology, Peking Union Medical College Hospital, Institute of Basic Medical Sciences & School of Basic Medicine, Chinese Academy of Medical Sciences, & Peking Union Medical College, Beijing, China.
Abstract:
This study investigated the activity of telomerase in peripheral blood mononuclear cells (PBMCs) in patients with laryngeal carcinoma. We examined proliferative response to phytohemagglutinin and expression of telomerase activity of PBMCs from 30 patients with laryngeal carcinoma and 17 healthy volunteers. Both proliferative and telomerase activity of [circled times]PBMCs in the patient group was lower than that in the healthy volunteer group. Telomerase activity may play a permissive role in cell division and clonal expansion of the immune cells in response to laryngeal squamous carcinoma. It may be related to lymph node metastasis of laryngeal cancer.
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