Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The yeast Pho80-Pho85 cyclin-CDK complex has multiple substrates.

Norman C Waters1, Janine P Knight, Caretha L Creasy

  • 1Department of Microbiology and Immunology, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, USA.

Current Genetics
|April 2, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic relatedness of Cambodian <i>Plasmodium falciparum</i> isolates is driven by geography and occupation.

medRxiv : the preprint server for health sciences·2026
Same author

Mutational analysis of an antimalarial drug target, <i>Pf</i>ATP4.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Screening of the Pandemic Response Box library identified promising compound candidate drug combinations against extensively drug-resistant Acinetobacter baumannii.

Scientific reports·2024
Same author

Strong positive selection biases identity-by-descent-based inferences of recent demography and population structure in Plasmodium falciparum.

Nature communications·2024
Same author

Strong Positive Selection Biases Identity-By-Descent-Based Inferences of Recent Demography and Population Structure in <i>Plasmodium falciparum</i>.

bioRxiv : the preprint server for biology·2023
Same author

First-in-human assessment of safety and immunogenicity of low and high doses of Plasmodium falciparum malaria protein 013 (FMP013) administered intramuscularly with ALFQ adjuvant in healthy malaria-naïve adults.

Vaccine·2022
Same journal

Is heptelidic (koningic) acid a microbial hormone that regulates secondary metabolism in the biocontrol fungus Trichoderma virens?

Current genetics·2025
Same journal

Loss of PDR3 alters metabolome in response to MCHM, a synthetic hydrotrope.

Current genetics·2025
Same journal

The CRISPR-cas repertoire of Kluyvera ascorbata: insights from genomic data.

Current genetics·2025
Same journal

Genome characterization of Acinetobacter species from the rice rhizosphere: a potential plant growth promoting rhizobacteria (PGPR).

Current genetics·2025
Same journal

Genomic surveillance of vancomycin-resistant Enterococcus faecium: a study on Resistome, Plasmidome, and mobilome profiling.

Current genetics·2025
Same journal

Epigenetic regulation of pathogenicity in fungi.

Current genetics·2025
See all related articles

The Pho85-Pho80 kinase complex regulates acid phosphatase in yeast. Phosphorylation of Pho80 at Ser234 is crucial for repressing acid phosphatase expression.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Yeast Genetics

Background:

  • The Pho85-Pho80 complex is a key regulator of phosphate metabolism in Saccharomyces cerevisiae.
  • This complex controls the expression of acid phosphatase, an enzyme responsive to phosphate levels.

Purpose of the Study:

  • To further characterize the Pho85-Pho80 kinase complex.
  • To identify substrates of the Pho85 protein kinase within this complex.
  • To elucidate the role of specific phosphorylation events in regulating acid phosphatase expression.

Main Methods:

  • Protein kinase assays to identify substrates.
  • Phosphorylation site mapping (mutagenesis) to pinpoint specific amino acid residues.
  • Analysis of gene expression to assess the functional impact of phosphorylation.

Related Experiment Videos

Main Results:

  • Pho80 cyclin and Pho81 CDK inhibitor were identified as substrates of Pho85.
  • Phosphorylation sites on Pho80 were identified at Ser234 and Ser267.
  • Phosphorylation of Pho80 at Ser234 is essential for forming an active kinase complex and repressing acid phosphatase expression.
  • Evidence suggests Pho85-mediated phosphorylation of Pho81 regulates its CDK inhibitor function.

Conclusions:

  • The control of acid phosphatase expression in yeast involves the phosphorylation of multiple regulatory components.
  • Pho85-mediated phosphorylation of Pho80 and Pho81 plays a critical role in this regulatory pathway.