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Updated: Aug 7, 2026

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry
Published on: May 28, 2014
A single nucleotide polymorphism identification assay for the genotypic characterisation of Neisseria meningitidis
C A Lowe1, M A Diggle, S C Clarke
1Scottish Meningococcus and Pneumococcus Reference Laboratory, North Glasgow University Hospitals Trust, Glasgow, UK.
Abstract:
The ability of matrix-assisted laser desorption-ionisation time-of-flight mass spectrometry (MALDI-TOF) to identify virulent clones of meningococci quickly and accurately is investigated. A single nucleotide polymorphism (SNP) within the fumC gene which differentiates between the hypervirulent ET-15 strain and other ET-37 complex strains is used to determine the usefulness of this method. In this study, MALDI-TOF proved to be a fast, effective alternative to traditional DNA sequencing for the identification of an individual nucleotide.
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