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[In vitro gene transfection by magnetic iron oxide nanoparticles and magnetic field increases transfection
Juan-juan Xiang1, Xin-min Nie, Jing-qun Tang
1Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Objective:
To evaluate the feasibility of using iron oxide nanoparticles as gene vector and the effect of magnetic field on efficiency of transfection.
Methods:
Iron oxide nanoparticles were prepared by alkaline precipitation of divalent and trivalent iron chloride. The surface of iron oxide nanoparticles was modified by self-assembled poly-L-lysine to form particle complexes (IONP-PLL). Transfection was determined by delivering reporter gene, PGL2-control encoding luciferase, to different cell lines using IONP-PLL as vector. The effect of magnetic field on efficiency of transfection was determined using Nd-Fe-B permanent magnet.
Results:
Foreign gene could be delivered to various cell lines by IONP-PLL and expressed with high efficiency, but the transfection efficiency and time course varied in the different cell lines studied. Magnetic field could enhance the efficiency of transfection by 5 - 10 fold.
Conclusion:
IONP-PLL can be used as a novel non-viral gene vector in vitro, which offers a basis for gene delivery in vivo.
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