Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Dissecting (CAC)5/(GTG)5 multilocus fingerprints from man into individual locus-specific, hypervariable components.

H Zischler1, C Kammerbauer, R Studer

  • 1Max-Planck-Institut für Psychiatrie, Martinsried, Germany.

Genomics
|August 1, 1992
PubMed
Summary

Researchers analyzed human DNA fingerprint components, focusing on (CAC)5/(GTG)5 repeats. They characterized these simple repetitive DNA sequences and their flanking regions for genetic and evolutionary insights.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In non-transformed cells Bak activates upon loss of anti-apoptotic Bcl-XL and Mcl-1 but in the absence of active BH3-only proteins.

Cell death & disease·2015
Same author

Association of TNFAIP3 and TNFRSF1A variation with multiple sclerosis in a German case-control cohort.

International journal of immunogenetics·2015
Same author

Fingerprinting plant genomes with oligonucleotide probes specific for simple repetitive DNA sequences.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Spotted Weimaraner dog due to de novo KIT mutation.

Animal genetics·2013
Same author

Genetics of toll like receptor 9 in ANCA associated vasculitides.

Annals of the rheumatic diseases·2013
Same author

Rapid genetic diversification within dog breeds as evidenced by a case study on Schnauzers.

Animal genetics·2012

Area of Science:

  • Molecular Biology
  • Human Genetics
  • Forensic Science

Background:

  • Multilocus DNA fingerprinting utilizes repetitive DNA sequences for individual identification.
  • The properties and genomic organization of specific repetitive elements like (CAC)5/(GTG)5 require detailed characterization.

Purpose of the Study:

  • To scrutinize individual components of human multilocus fingerprints derived from (CAC)5/(GTG)5 oligonucleotides.
  • To characterize the properties and genomic localization of these simple repetitive DNA sequences.
  • To assess their utility in individualization and linkage studies.

Main Methods:

  • Cloning and propagation of recombinant simple repetitive DNA in prokaryotic hosts.
  • Characterization of isolated locus-specific probes for formal and population genetic properties.

Related Experiment Videos

  • Chromosomal localization studies (chromosomes 8, 9, 11, 22) and analysis of repeat flanking sequences.

    • Main Results:

    • Detailed characterization of (CAC)5/(GTG)5 oligonucleotide components in human DNA fingerprinting.
    • Successful cloning and analysis of recombinant simple repetitive DNA propagation.
    • Identification of locus-specific probes with defined genetic properties and chromosomal localizations.
    • Analysis of flanking sequences revealed coding potential and evolutionary conservation.
    • Absence of classical minisatellites near these hypervariable repeats.

    Conclusions:

    • The characterized single-copy probes serve as valuable complements to oligonucleotide-based multilocus fingerprinting.
    • These probes offer practical applications in human individualization and population genetics.
    • Understanding the genomic organization and evolutionary aspects of these repeats enhances their utility.