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Updated: Aug 25, 2026
A Multi-detection Assay for Malaria Transmitting Mosquitoes
Published on: February 28, 2015
Design and evaluation of a specific primer pair for the diagnosis and identification of Acanthamoeba polyphaga
Antonio Ortega-Rivas1, Jacob Lorenzo-Morales, Enrique Martinez-Carretero
1Department of Parasitology, Faculty of Pharmacy, University of La Laguna, Avda. Francisco Sánchez s/n, C.P. 38271, La Laguna, Tenerife, Spain. aortega@ull.es
Abstract:
Random amplified polymorphism DNA (RAPD) is a useful tool for species identification. The obtained band patterns can be used for specific primer pair design that may be useful for species diagnosis. In this study, a distinctive a 962-bp band in A. polyphaga band patterns was found, by using the OPC20 primer (ACTTCGCCAC). The DNA fragment was used to design a specific primer pair that was useful for the identification of different isolates as A. polyphaga species. A case of A. polyphaga in disseminated acanthamoebiasis affecting mesenteric nodes is also reported.
