Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Eucaryotic DNA replication complex: study of structure and function using the affinity modification technique.

D Yu Khlimankov1, N I Rechkunova, O I Lavrik

  • 1Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, Russian Academy of Sciences, Novosibirsk 630090, Russia.

Biochemistry. Biokhimiia
|April 6, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The effect of dimeric bisbenzimidazoles on the activity of DNA repair enzymes TDP1, TDP2, PARP1 and PARP2.

Vavilovskii zhurnal genetiki i selektsii·2026
Same author

[Amidothiazole Derivatives of (+)-Usnic Acid Effectively Inhibit TDP1 and Sensitize Tumor Cells to the Effects of Topotecan].

Molekuliarnaia biologiia·2025
Same author

Comparative analysis of the primary structure and production of recombinant poly(ADP-ribose)polymerase 1 of long-lived Heterocephalus glaber.

Vavilovskii zhurnal genetiki i selektsii·2024
Same author

Non-Catalytic Domains of DNA Polymerase λ: Influence on Enzyme Activity and Its Regulation.

Doklady. Biochemistry and biophysics·2023
Same author

[Effect of Usnic Acid-Derived Tyrosyl-DNA Phosphodiesterase 1 Inhibitor Used as Monotherapy or in Combination with Olaparib on Transplanted Tumors In Vivo].

Molekuliarnaia biologiia·2023
Same author

[Poly(ADP-Ribose) Polymerases 1 and 2: Classical Functions and Interaction with New Histone Poly(ADP-Ribosyl)ation Factor HPF1].

Molekuliarnaia biologiia·2023
Same journal

Multidirectional Effects of SARS-CoV-2 Coronavirus Proteins on Amyloid Transformation of Alpha-Synuclein.

Biochemistry. Biokhimiia·2026
Same journal

Dependence of Antioxidant and Transcriptional Responses to Periodic Hypoxia on Sex and Age in Rats.

Biochemistry. Biokhimiia·2026
Same journal

Effect of the PPARγ Agonist Pioglitazone on Rat Behavior and Expression of Epileptogenesis-Related Genes during the Latent Phase of the Lithium-Pilocarpine Model.

Biochemistry. Biokhimiia·2026
Same journal

A Conjugate of Aminoadamantane and Tetrahydro-γ-Carboline Inhibits Accumulation of Mutant α-Synuclein A53T in the Cellular Model of Proteinopathy.

Biochemistry. Biokhimiia·2026
Same journal

Immunoinflammatory Markers in Patients with Affective Disorders: Genetic Polymorphisms, Peripheral Cytokine Levels, and Expression of IL-1β, IL-13, TNF-β, and TGF-α in Peripheral Blood Mononuclear Cells.

Biochemistry. Biokhimiia·2026
Same journal

Method for Individual Assessment of Human Cerebral Cortex Activity by a Combined Use of Magnetic Resonance Spectroscopy of Glutamate and BOLD Signal Method.

Biochemistry. Biokhimiia·2026
See all related articles

Affinity modification effectively studies the eukaryotic DNA replication complex, revealing protein interactions. This method overcomes limitations of X-ray diffraction for dynamic, multi-component systems.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Structural Biology

Background:

  • Eukaryotic DNA replication is a key area in molecular biology and biochemistry.
  • Studying replication complexes involves analyzing DNA polymerases, enzymes, and protein factors.
  • X-ray diffraction analysis provides structural insights but has limitations for certain proteins and dynamic systems.

Purpose of the Study:

  • To review and analyze data from affinity modification studies on the eukaryotic replication complex.
  • To highlight affinity modification as a method for studying complex biological systems.

Main Methods:

  • Affinity modification is presented as an alternative to X-ray diffraction.
  • This technique is suitable for studying individual enzymes, their complexes, and multi-component systems like DNA replication and repair complexes.

Related Experiment Videos

  • It allows for the investigation of dynamic interactions within enzymatic systems.

    • Main Results:

    • Affinity modification provides valuable data on the structure-functional organization of supramolecular structures.
    • It is applicable to systems with numerous interacting proteins and nucleic acids.
    • The approach overcomes limitations of X-ray diffraction for studying proteins not amenable to isolation in sufficient quantities.

    Conclusions:

    • Affinity modification is a powerful approach for studying the eukaryotic replication complex.
    • It offers advantages over X-ray diffraction for analyzing dynamic and complex biological systems.
    • This method is crucial for understanding the intricate interactions within DNA replication and repair machinery.