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Selective activation of protein kinase C isoforms by angiotensin II in neuroblastoma X glioma cells

Karen J Greenland1, Amal K Mukhopadhyay

  • 1Institute for Hormone and Fertility Research, University of Hamburg, Grandweg 64, Hamburg 22529, Germany. karen.greenland@mcri.edu.au

Molecular and Cellular Endocrinology
|April 6, 2004
PubMed

Insights

Angiotensin II differentially activates protein kinase C (PKC) isoforms in brain cells. This study identifies PKC alpha and zeta as key players in Angiotensin II signaling within the nervous system.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Cell Signaling

Background:

  • Angiotensin II (AII) exerts diverse effects in various tissues through differential activation of protein kinase C (PKC) isoforms.
  • The specific PKC isoforms involved in AII's brain-specific actions remain largely undefined.

Purpose of the Study:

  • To identify the PKC isoforms activated by AII in the NG108-15 neuroblastoma X glioma hybrid cell line.
  • To investigate the differential sensitivity of these isoforms to AII stimulation in both undifferentiated and differentiated cells.

Main Methods:

  • Western blot analysis was employed to detect and quantify PKC isoform expression and activation.
  • NG108-15 cells, expressing AT1 and AT2 receptors, were used to examine AII-induced PKC activation.

Main Results:

  • NG108-15 cells expressed PKC alpha, delta, and zeta isoforms.
  • AII induced rapid, transient activation of PKC alpha in undifferentiated cells.
  • Both PKC alpha and zeta isoforms were responsive to AII in differentiated cells, demonstrating differential activation patterns.

Conclusions:

  • The study reveals differential activation of PKC isoforms by AII in NG108-15 cells.
  • Evidence suggests the involvement of PKC alpha and zeta isoforms in AII-mediated effects within the brain.

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