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Related Experiment Videos

Injectable controlled release formulations incorporating protein crystals.

Sergey Pechenov1, Bhami Shenoy, Mark X Yang

  • 1Department of Pharmaceutics, Altus Biologics Inc., 625 Putnam Ave., Cambridge, MA 02139, USA.

Journal of Controlled Release : Official Journal of the Controlled Release Society
|April 6, 2004
PubMed
Summary

Protein crystals offer a novel approach for injectable controlled-release systems. These crystalline protein depots overcome stability issues, enabling sustained protein delivery without initial drying.

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Area of Science:

  • Biomaterials Science
  • Drug Delivery Systems
  • Protein Crystallography

Background:

  • Developing injectable, in situ formable controlled-release gel systems for proteins presents significant challenges.
  • Protein instability in organic solvents and the requirement for initial drying hinder fabrication.
  • Existing methods often compromise protein integrity and loading efficiency.

Purpose of the Study:

  • To develop and characterize injectable controlled-release systems using protein crystals.
  • To evaluate the feasibility of incorporating protein crystals into in situ formable gels.
  • To assess the stability and release profiles of proteins in crystalline form within these systems.

Main Methods:

  • Crystallization of alpha-amylase from Aspergillus oryzae.

Related Experiment Videos

  • Suspension of protein crystals in poly(DL-lactide-co-glycolide) (PLGA) in acetonitrile or sucrose acetate isobutyrate (SAIB) with ethanol.
  • Characterization of protein loading, release profiles, and long-term stability.

    • Main Results:

    • Protein crystals were successfully incorporated into in situ formable gels without prior drying.
    • Crystalline proteins demonstrated stability in organic solvents and at water/organic solvent interfaces.
    • High protein loading (>30%) was achieved, and release profiles were modulated by crystal morphology.
    • Crystalline amylase exhibited superior long-term stability compared to amorphous forms.

    Conclusions:

    • Protein crystals are a promising strategy for developing stable, injectable, in situ formable protein depot systems.
    • This approach overcomes key limitations associated with conventional protein-based controlled-release formulations.
    • Protein crystallinity enhances stability and facilitates high loading in injectable delivery systems.