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Published on: January 17, 2016
Tbx18 and boundary formation in chick somite and wing development
Mikiko Tanaka1, Cheryll Tickle
1Division of Cell and Developmental Biology, Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, UK. mtanaka@uoneiro.uoregon.edu
Chicken Tbx18 gene expression is crucial for forming mesodermal boundaries. It controls somite segmentation and influences limb bud development by regulating fissure formation and anterior extension.
Area of Science:
- Developmental Biology
- Molecular Genetics
- Embryology
Background:
- The Tbx gene family plays vital roles in embryonic development.
- Tbx18's precise function in somite and limb development requires further elucidation.
Purpose of the Study:
- To investigate the role of chicken Tbx18 in mesodermal boundary formation.
- To understand Tbx18's function during somite segmentation and limb development.
Main Methods:
- Analysis of Tbx18 expression patterns in developing chicken embryos.
- Overexpression and transplantation experiments to assess functional impact.
- Examination of somite maturation and limb bud morphology.
Main Results:
- Tbx18 is transiently expressed in axial mesenchyme during somite segmentation.
- Overexpression and transplantation studies indicate Tbx18 controls fissure formation in maturing somites.
- Ectopic Tbx18 in lateral plate mesoderm causes anterior wing bud extension.
Conclusions:
- Tbx18 is essential for establishing mesodermal boundaries in paraxial and lateral plate mesoderm.
- The gene regulates the formation of inter-somite boundaries and the wing/non-wing boundary.
- Tbx18 acts as a key regulator in embryonic patterning and morphogenesis.
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