Identification of hydroxy fatty acids by liquid chromatography-atmospheric pressure chemical ionization mass
Lory Z Santiago-Vázquez1, Laura D Mydlarz, James G Pavlovich
1Department of Ecology, Evolution and Marine Biology, University of California, Santa Barbara, CA 93106, USA.
Abstract:
Hydroxy fatty acids from Euglena gracilis were identified by reverse-phase high performance liquid chromatography coupled to a mass spectrometer run in atmospheric pressure chemical ionization positive ion mode. These metabolites were converted to methyl esters to improve stability and chromatographic properties. A detection limit of 20 pg/microl per injection was determined for 5-HETE methyl ester based on the signal to noise ratio of the m/z 317 ion which corresponds to the loss of a hydroxyl group (M-17) and the major fragment in all HETE methyl esters studied. This is the first report for these metabolites in E. gracilis.
