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Harnessing natural transformation in Actinobacillus pleuropneumoniae: a simple method for allelic replacements

Janine T Bossé1, John H E Nash, J Simon Kroll

  • 1Molecular Infectious Disease Group, Department of Paediatrics, Faculty of Medicine, Imperial College London, St. Mary's Campus, Norfolk Place, London W2 1PG, UK. j.bosse@imperial.ac.uk

FEMS Microbiology Letters
|April 6, 2004
PubMed

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In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...