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Related Experiment Videos

A multipurpose instrument for quantitative intravital microscopy.

A Toth1, M E Tischler, M Pal

  • 1Department of Physiology, University of Arizona, Tucson 85724.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|July 1, 1992
PubMed
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A novel in vivo microscope system enables simultaneous measurement of tissue fluorescence and microcirculatory parameters like oxygen saturation. This advanced tool accurately assesses cellular and vascular function in real-time.

Area of Science:

  • Biomedical Engineering
  • Microscopy
  • Physiology

Background:

  • Assessing parenchymal cell and microcirculatory function requires sophisticated in vivo measurement techniques.
  • Existing methods may lack the capability for simultaneous, high-resolution analysis of both cellular metabolism and vascular dynamics.

Purpose of the Study:

  • To develop and validate an advanced in vivo microscope system for simultaneous measurement of tissue fluorescence and microcirculatory parameters.
  • To enable precise, site-specific analysis of cellular and vascular function in biological tissues.

Main Methods:

  • Development of a multi-wavelength in vivo microscope system with a computer-controlled stage and data acquisition.
  • Integration of confocal optics for precise definition of the monitored tissue area (18-30 microns).

Related Experiment Videos

  • Simultaneous video imaging of microcirculatory vessels and spectrophotometric determination of oxygen saturation and hematocrit.

    • Main Results:

    • The system demonstrated good agreement between NADH fluorescence at 450 nm and NADH concentration in vitro and in skeletal muscle.
    • In vitro tests confirmed suitable accuracy for simultaneous spectrophotometric determination of oxygen saturation and hematocrit in microvessels.
    • The instrument allows for rapid, repeated measurements from multiple tissue sites.

    Conclusions:

    • The developed in vivo microscope system offers a versatile platform for simultaneous assessment of parenchymal cell and microcirculatory function.
    • Its modular design and advanced capabilities facilitate diverse research applications in tissue physiology and disease.
    • This technology advances the potential for real-time, in-depth analysis of microvascular and cellular metabolic states.