Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural basis of protein-protein interactions.

Robert C Liddington1

  • 1The Burnham Institute, La Jolla, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|April 6, 2004
PubMed
Summary

Protein interactions control cellular signaling. Structural studies reveal diverse protein complex types, including domain-domain and domain-peptide interactions, crucial for signal transduction regulation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dual function of Zika virus NS2B-NS3 protease.

PLoS pathogens·2023
Same author

PLEKHA7 signaling is necessary for the growth of mutant KRAS driven colorectal cancer.

Experimental cell research·2021
Same author

Structural basis for the association of PLEKHA7 with membrane-embedded phosphatidylinositol lipids.

Structure (London, England : 1993)·2021
Same author

Assembly and Function of the Anthrax Toxin Protein Translocation Complex.

Sub-cellular biochemistry·2020
Same author

Correction: An Inhibitor of the Pleckstrin Homology Domain of CNK1 Selectively Blocks the Growth of Mutant KRAS Cells and Tumors.

Cancer research·2019
Same author

An Inhibitor of the Pleckstrin Homology Domain of CNK1 Selectively Blocks the Growth of Mutant KRAS Cells and Tumors.

Cancer research·2019

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Biochemistry

Background:

  • Protein-protein interactions are fundamental to cellular signaling pathways.
  • Experimentally determined structures offer insights into protein recognition principles.
  • Signal transduction relies on diverse protein complex formations.

Purpose of the Study:

  • To explore the structural diversity of protein complexes in signal transduction.
  • To categorize protein-protein interactions based on structural characteristics.
  • To elucidate the mechanisms of regulation in signaling complexes.

Main Methods:

  • Analysis of experimentally determined structures of protein complexes.
  • Classification of complexes into domain-domain and domain-peptide types.
  • Investigation of intramolecular domain interactions in multidomain proteins.

Main Results:

  • Identified two primary classes of protein complexes: domain-domain and domain-peptide.
  • Highlighted the significant diversity in interfacial size and nature of these interactions.
  • Demonstrated the role of multidomain proteins and intramolecular interactions in regulating binding functions.

Conclusions:

  • Protein complex structures exhibit remarkable diversity, impacting cellular signaling.
  • Understanding these structures is key to deciphering signal transduction mechanisms.
  • Steric and allosteric mechanisms underlie the regulation of protein interactions in signaling.

Related Experiment Videos